DYSREGULATION OF PLACENTAL MIRNA IN MATERNAL OBESITY IS ASSOCIATED WITH PRE-AND POST-NATAL GROWTH.

DYSREGULATION OF PLACENTAL MIRNA IN MATERNAL OBESITY IS ASSOCIATED WITH PRE-AND POST-NATAL GROWTH.

English
Grup d'investigació: 

 

Human placenta exhibits a specific miRNA expression pattern. Some of these miRNAs are dysregulated in pregnancy disorders like preeclampsia and intrauterine growth restriction, and are potential biomarkers for these pathologies.

OBJECTIVE:

To study the placental miRNA profile in pregnant women with pregestational (preOB) or gestational obesity (gestOB) and explore the associations of placental miRNAs dysregulated in maternal obesity with pre- and post-natal growth.

METHODS:

TaqMan Low-Density Arrays (TLDAs) and real-time PCR were used to profile the placental miRNAs in 70 pregnant women (20 preOB, 25 gestOB and 25 control). Placentas and newborns were weighed at delivery and at 1, 4 and 12 month of life.

RESULTS:

8 miRNAs were decreased in placentas from preOB or gestOB (miR-100, miR-1269, miR-1285, miR-181, miR-185, miR-214, miR-296 and miR487) (all p <0.05). Among them, miR-100, miR-1285, miR-296 and miR-487 associated with maternal metabolic parameters (all p<0.05) and were predictors of lower birth weight (all p<0.05 and R2>30%) and increased postnatal weight gain (all p<0.05 and R2>20%). In silico analysis showed that these miRNAs were related to cell proliferation and insulin signalling pathways. miR-296 was also present in plasma samples and associated with placental expression and with pre- and postnatal growth parameters (all p <0.05).

CONCLUSIONS:

We identified a specific placental miRNA profile in maternal obesity. The dysregulation of placental obesity-associated miRNAs may participate in the mediation of growth promoting effects of maternal obesity on the offspring and could be used as early markers of pre- and postnatal growth.

Quartile: 
1